森林立地55（2），139～143 2013
Jpn J For Environ
55 (2), 139―143, 2013
Responses of microbial community to soil warming
in warm-temperate evergreen broad-leaved forests
Xin Wang1, Takayuki Nakatsubo1,
Akiko Sasaki1,
Shinpei Yoshitake2, Naishen Liang3
and Kaneyuki Nakane1
1Department of Environmental Dynamics and Management,
Graduate School of Biosphere Science, Hiroshima University
2Takayama Experimental Field Station, River Basin
Research Center, Gifu University
3CGER, National Institute for Enviromental studies,
Tskuba
To elucidate the effect of climate warming on the soil heterotrophic microbial community in warm-temperate, evergreen broad-leaved forests, we conducted a soil-warming experiment in a secondary forest located in the city of Higashi-Hiroshima in western Japan. We established ten trench plots (1 m × 1 m) with root barriers to prevent root regrowth in the forest. The plots were divided into warming and control treatments. Infrared heaters were used to increase the soil temperature of the warming plots by about 2.5℃ for three years. We used phospholipid fatty acid (PLFA) analysis to examine the composition of the soil heterotrophic microbial community. There were no significant differences in the total content of PLFAs (TotPLFAs) and fungal PLFAs (FungPLFAs) between the warming and control plots. However, warming caused an increase in the amount of bacterial PLFAs (BactPLFAs), the result being a lower ratio of FungPLFAs to BactPLFAs (F/B ratio) in the warming plots. In addition, PLFAs characteristic of gram-negative bacteria increased in the warming plots. The results indicated that the soil heterotrophic microbial community in this warm-temperate, evergreen broad-leaved forest was sensitive to climate warming.
Key Words : evergreen broad-leaved forest, microbial community composition, phospholipid fatty acid (PLFA), soil warming, warm-temperate zone
王新・中坪孝之・佐々木晶子・吉竹晋平・梁乃申・中根周歩：暖温帯常緑広葉樹林における土壌加温に対する微生物群集の反応．森林立地55：139-143, 2013